Dahlén G, Fabricius L, Holm S E, Möller A J
Scand J Dent Res. 1982 Oct;90(5):338-44. doi: 10.1111/j.1600-0722.1982.tb00746.x.
Root canals of 49 teeth with necrotic pulp tissue in five monkeys were infected with Streptococcus faecalis ss liquefaciens, Actinomyces bovis, Fusobacterium nucleatum, Peptostreptococcus anaerobius, and Bacteroides oralis in various combinations. After 6 months the root canals and the periapical tissues were subjected to radiographic, bacteriologic, and histologic examinations. Signs of periapical inflammation were radiographically registered in 41 teeth, most frequently in teeth inoculated with a mixed flora. In 11 out of 16 teeth infected separately with Strep. faecalis ss liquefaciens, apical periodontitis was observed. Sera from the monkeys prior to and after the experimental inoculation were analyzed by means of gel diffusion, hemagglutination (HA), and complement binding test for antibodies against different antigen preparations of the homologous bacterial strains used for the inoculation. Detectable antibodies were seen with antigens of B. oralis in all five monkeys. Agglutinating antibodies were demonstrated with lipopolysaccharide-antigen of B. oralis in titers between 1:40 and 1:320. A marked reduction of the antibody level against lipopolysaccharide (LPS) antigen after mercaptoethanol treatment indicated that a main part of the antibodies registered was of IgM-class. The study shows that certain antigens of bacterial origin from infected root canals, while affecting the periapical tissues, also stimulate the production of circulating antibodies.
用粪链球菌液化亚种、牛放线菌、具核梭杆菌、厌氧消化链球菌和口腔拟杆菌的不同组合感染了5只猴子中49颗牙髓坏死牙齿的根管。6个月后，对根管和根尖周组织进行了影像学、细菌学和组织学检查。41颗牙齿在影像学上显示有根尖周炎症迹象，最常见于接种混合菌群的牙齿。在分别感染粪链球菌液化亚种的16颗牙齿中，有11颗观察到根尖周炎。通过凝胶扩散、血凝（HA）和补体结合试验分析了实验接种前后猴子血清中针对接种所用同源菌株不同抗原制剂的抗体。在所有5只猴子中均检测到针对口腔拟杆菌抗原的抗体。用口腔拟杆菌的脂多糖抗原检测到凝集抗体，效价在1:40至1:320之间。巯基乙醇处理后，针对脂多糖（LPS）抗原的抗体水平显著降低，表明所检测到的抗体主要为IgM类。该研究表明，来自感染根管的某些细菌源性抗原在影响根尖周组织的同时，也刺激循环抗体的产生。
