Bromage T G
Am J Orthod. 1982 Apr;81(4):314-21. doi: 10.1016/0002-9416(82)90218-4.
The histologic research on craniofacial remodeling is briefly summarized. A new methodologic approach using the scanning electron microscope (SEM) and high-resolution replicas of craniofacial bone is evaluated. Two maxillae were chosen for illustrative purposes. The specimens were replicated and prepared for routine SEM examination. In addition, a grid was applied to the replicas so that the bone growth activity states could be mapped on a coordinate system. The topographic ("T") principle is introduced as a precedent for discriminating remodeling bone growth activity states with the SEM. These activity states in vivo specify characteristic microscopic surface topographies. The three distinctive surfaces are resorptive, depository, and resting, which are mapped on a coordinate system. Results obtained are similar to those of histologic studies. The primary advantage of the SEM/replica technique is that it does not damage the specimen. This feature will facilitate more extensive investigations of craniofacial remodeling. The time and financial investments for the SEM/replica technique are significantly less than the histologic technique in the investigation of similar material. The SEM/replica technique, however, cannot be used to study nonexternal surfaces and evidence of remodeling contained within the bone cortex.
本文简要总结了颅面重塑的组织学研究。评估了一种使用扫描电子显微镜（SEM）和颅面骨高分辨率复制品的新方法。为说明目的选择了两块上颌骨。对标本进行复制并准备进行常规SEM检查。此外，在复制品上应用了一个网格，以便可以在坐标系上绘制骨生长活动状态。引入地形学（“T”）原理作为用SEM区分重塑骨生长活动状态的先例。体内的这些活动状态指定了特征性的微观表面形貌。三种独特的表面是吸收性、沉积性和静止性的，它们被绘制在坐标系上。获得的结果与组织学研究的结果相似。SEM/复制品技术的主要优点是它不会损坏标本。这一特性将有助于对颅面重塑进行更广泛的研究。在研究类似材料时，SEM/复制品技术的时间和资金投入明显少于组织学技术。然而，SEM/复制品技术不能用于研究非外表面以及骨皮质内包含的重塑证据。
