Interrelationship between delayed hypersensitivity and macrophage resistance to infection by influenza virus.

Interaction between the influenza virus and the sensitized cells, depending on the number of animal immunizations, was studied on a model of mouse peritoneal macrophages. The maximal suppression of the macrophage migration was observed in 2 weeks in 21% of mice immunized once, and in 35% of mice immunized twice. Three hours after the infection of the macrophage culture with the influenza virus in a dose of 10 EID50 there were revealed in the cells obtained from mice immunized once 70%, and from mice immunized twice 8% of the infectious virus in comparison with the intact macrophages. There was a direct relationship between the delayed hypersensitivity determined in the macrophage inhibition migration test and the macrophage resistance to the infection with the influenza virus.