[持续培养的伤寒杆菌的免疫原性和抗原性]

[Immunogenic and antigenic properties of continuously cultured typhoid bacteria].

作者信息

Zhdanova L G, Gruber I M, Mashilova G M, Savranskaia S Ia

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1978 Jul(7):99-4.

Abstract

It was shown that in continuous cultivation of typhoid bacilli, by changing the rate of dilution and the regimens of carbohydrate and oxygen supply it was possible to determine conditions under which the most highly immunogenic vaccines were obtained and the maximal synthesis of antigens was observed. The regimen optimal for obtaining the highly immunogenic typhoid vaccines (D = 0.4 h-1; So = 5 g/l; pO2 = 50%) coincided with the regimen at which the maximal O-antigen synthesis was seen, and failed to coincide with the regimens necessary for the greatest synthesis of Vi- and H-antigens.

摘要

结果表明，在伤寒杆菌的连续培养中，通过改变稀释速率以及碳水化合物和氧气供应方案，可以确定获得免疫原性最强的疫苗以及观察到抗原最大合成量的条件。获得高免疫原性伤寒疫苗的最佳方案（D = 0.4 h-1；So = 5 g/l；pO2 = 50%）与观察到最大O抗原合成的方案一致，但与Vi抗原和H抗原最大合成所需的方案不一致。