利用高场¹³C核磁共振对鸡蛋清溶菌酶中单个羧基的观察。
Observation of individual carboxyl groups in hen egg-white lysozyme by use of high field 13C-nuclear magnetic resonance.
作者信息
Shindo H, Cohen J S
出版信息
Proc Natl Acad Sci U S A. 1976 Jun;73(6):1979-83. doi: 10.1073/pnas.73.6.1979.
Several of the carboxyl carbon atom resonances of hen egg-white lysozyme (mucopeptide N-acetylmuramoyl hydrolase, EC 3.2.1.17) have been resolved by 13C-nuclear magnetic resonance (NMR) at 68 MHz. The change in chemical shift of the carboxyl carbon atom resonances, as a function of pH, has enabled the distinction of these resonances against the background of many nontitrating carbonyl group resonances. Several apparent microscopic ionization constants have been determined from the carboxyl group NMR titration curves, and possible assignments are discussed. Preliminary experiments were carried out in the presence of cobaltous ion, and selective shifts of several resonances were observed. Our results indicate the possibility of the direct observation of a wide range of single functional groups of proteins in solution by NMR techniques.
通过68兆赫的碳-13核磁共振（NMR）已分辨出鸡蛋清溶菌酶（粘肽N-乙酰胞壁酰水解酶，EC 3.2.1.17）的几个羧基碳原子共振峰。羧基碳原子共振峰的化学位移随pH值的变化，使得在许多非滴定羰基共振峰的背景下能够区分这些共振峰。从羧基NMR滴定曲线确定了几个表观微观电离常数，并讨论了可能的归属。在钴离子存在下进行了初步实验，观察到几个共振峰的选择性位移。我们的结果表明，通过NMR技术直接观察溶液中蛋白质广泛的单官能团是可能的。
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