Gower D B, Cooke G M, Ferguson S E
FEBS Lett. 1982 Dec 27;150(2):507-10. doi: 10.1016/0014-5793(82)80800-4.
Steroid sulphotransferase activities for 5 alpha-androst-16-en-3 beta-ol and pregnenolone in porcine liver cytosol have been assayed using 3'-phosphoadenosine-5'-phospho[35S]sulphate as sulphate donor. 5 alpha-Androst-16-en-3 beta-ol sulphotransferase activity was obtained from porcine liver cytosol by gel filtration chromatography; activity was linear with time up to about 5 min., the optimum pH was near 8.0 and optimum temperature 37 degrees C. Pregnenolone sulphotransferase activity was partially purified from porcine liver cytosol using DEAE-cellulose chromatography with an ionic gradient of KC1. This enzyme activity was linear with time up to 10 min and had optimum pH and temperature of 8.0 and 37 degrees C, respectively.
使用3'-磷酸腺苷-5'-磷酸[35S]硫酸盐作为硫酸盐供体，测定了猪肝胞液中5α-雄甾-16-烯-3β-醇和孕烯醇酮的类固醇磺基转移酶活性。通过凝胶过滤色谱法从猪肝胞液中获得了5α-雄甾-16-烯-3β-醇磺基转移酶活性；在大约5分钟内，活性与时间呈线性关系，最适pH接近8.0，最适温度为37℃。使用含KCl离子梯度的DEAE-纤维素色谱法从猪肝胞液中部分纯化了孕烯醇酮磺基转移酶活性。该酶活性在长达10分钟的时间内与时间呈线性关系，最适pH和温度分别为8.0和37℃。
