Miake Y, Yanagisawa T, Takuma S
J Biol Buccale. 1982 Dec;10(4):319-30.
Young Wistar male rats were injected intravenously with 2 mg/kg of vinblastine sulfate. After three, six, 12, 24 and 48 hours, the upper incisors were dissected out of the animals, and their young odontoblasts were observed in an electron microscope. Three to six hours after the injection, the regular polarity of the nuclei and the cytoplasmic organelles were dramatically changed following a decrease in the number of microtubules. Disturbance in protein synthesis was suggested at this stage by unusual shapes and the arrangement of the rough endoplasmic reticulum and loss of the polysomal arrangement of the ribosomes attached to the reticulum. After more than 12 hours, accumulation of secretion granules and the occurrence of bodies, containing a few strands of collagen-like fibrils, were frequently seen in various portions of the cells, indicating retarded export of the secretion substance. Occurrence of a number of unusually short fibrils in predentin was another prominent feature noticed in later stages. The fibrils were about 3000A long by 2000A or 600A wide, and many of the 2000A wide fibrils were characterized by a banded structure which resembled the previously reported SLS collagen. The 600A wide fibrils were usually banded with about 600A periodicity.
将2毫克/千克的硫酸长春碱静脉注射到年轻的雄性Wistar大鼠体内。在注射后3、6、12、24和48小时，从动物身上取出上切牙，在电子显微镜下观察其年轻的成牙本质细胞。注射后3至6小时，微管数量减少后，细胞核和细胞质细胞器的正常极性发生了显著变化。此时，粗面内质网的异常形状和排列以及附着在网状结构上的核糖体多聚体排列的丧失表明蛋白质合成受到干扰。12小时后，在细胞的各个部分经常可以看到分泌颗粒的积累以及含有几束胶原样纤维的小体的出现，这表明分泌物质的输出受阻。前期牙本质中出现许多异常短的纤维是后期观察到的另一个突出特征。这些纤维长约3000埃，宽2000埃或600埃，许多宽2000埃的纤维具有带状结构，类似于先前报道的SLS胶原。宽600埃的纤维通常具有约600埃的周期性带状结构。
