Xu B
Am J Chin Med. 1981 Winter;9(4):268-76. doi: 10.1142/s0192415x81000354.
The influence of homoharringtonine, hydroxycamptothecin and lycobetaine on the cell cycle progression of murine erythroleukemia cells was studied by using flow microfluorometry (FMF) technique and centrifugal elutriation to obtain specific fractions of the cell cycle. FMF histogram analysis showed that homoharringtonine could strongly arrest cells in the G1 phase of the immediate cell cycle. This effect was more pronounced and persisted longer with G1 cells than with S or G2 cells. Hydroxycamptothecin mainly delayed the progression of S cells of the subsequent cell cycle (daughter cells). Lycobetaine caused a marked accumulation of G2 cells. These 3 compounds possess a relatively specific action on cell progression through the cell cycle. Homoharringtonine and hydroxycamptothecin can inhibit the cell proliferation of murine erythroleukemia cells (MELC) at low concentrations (ng/ml) whereas lycobetaine, cantharidin and oxalysine are less potent. Among them only hydroxycamptothecin had a weak activity to induce MELC differentiation. These results may provide some basic knowledge for designing new protocols of the combination treatment of neoplastic diseases.
运用流式显微荧光测定法（FMF）技术以及离心淘析法获取细胞周期的特定部分，研究高三尖杉酯碱、羟基喜树碱和石蒜碱对小鼠红白血病细胞周期进程的影响。FMF直方图分析显示，高三尖杉酯碱能够强烈阻滞细胞于紧邻细胞周期的G1期。这种作用在G1期细胞中比在S期或G2期细胞中更为显著且持续时间更长。羟基喜树碱主要延迟后续细胞周期（子细胞）中S期细胞的进程。石蒜碱导致G2期细胞明显积累。这3种化合物对细胞通过细胞周期的进程具有相对特异性作用。高三尖杉酯碱和羟基喜树碱能够在低浓度（纳克/毫升）时抑制小鼠红白血病细胞（MELC）的细胞增殖，而石蒜碱、斑蝥素和草赖氨酸的作用较弱。其中只有羟基喜树碱具有较弱的诱导MELC分化的活性。这些结果可能为设计肿瘤疾病联合治疗的新方案提供一些基础知识。
