体外髓鞘成熟异常：脑苷脂的作用。
Abnormal myelin maturation in vitro: the role of cerebrosides.
作者信息
Bradbury K
出版信息
Adv Exp Med Biol. 1978;100:171-8. doi: 10.1007/978-1-4684-2514-7_12.
Myelinating neonatal rat cerebellar explants were maintained for up to 130 days in vitro. Myelin fractions were extracted from explants of different ages and purified by density gradient centrifugation. Three fractions obtained were termed "light myelin", "heavy myelin" and "membrane fraction" and were deficient in glycolipids compared to myelin fractions prepared from 15-day-old rat cerebellum. The culture myelin has an apparently normal ultrastructure but may not be as stable as myelin with a normal glycolipid composition.
新生大鼠小脑髓鞘形成外植体在体外培养长达130天。从不同年龄的外植体中提取髓鞘组分，并通过密度梯度离心进行纯化。得到的三个组分分别称为“轻髓鞘”、“重髓鞘”和“膜组分”，与从15日龄大鼠小脑中制备的髓鞘组分相比，它们的糖脂含量较低。培养的髓鞘具有明显正常的超微结构，但可能不如具有正常糖脂组成的髓鞘稳定。
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