Gorczynski R M, Kennedy M J, MacRae S, Steele E J, Cunningham A J
J Immunol. 1980 Feb;124(2):590-6.
B cells, like T cells, show genetic restrictions in their interactions with other cells. B cells from (C3H/HeJ X C57BL/6J)F1 mice were stimulated with foreign antigen (sheep erythrocytes, SRBC) in parental-strain irradiated hosts. They could be subsequently activated to antibody formation in vitro by SRBC presented on cells of the same parental type, and much less by SRBC on cells of the other parental type. The restriction was seen whether antigen was presented on macrophages or by activated T cells. Experiments with congenic mice showed that the restriction was controlled by the MHC locus. Restriction of F1 B cells to responsiveness against either parental haplotype was much more readily induced in bone marrow than in splenic populations. This restriction could be broken down by giving the irradiated host a second injection of antigen 3 weeks after the first; this probably reflects "reeducation" of the F1 B cells by SRBC associated with donor, F1-type macrophages. Normal unprimed spleen B cells also showed a strong preference for activation by SRBC in association with syngeneic activated T cells.
与T细胞一样，B细胞在与其他细胞相互作用时也表现出基因限制。将（C3H/HeJ×C57BL/6J）F1小鼠的B细胞在亲本品系经辐照的宿主体内用外来抗原（绵羊红细胞，SRBC）刺激。随后，它们可以被相同亲本品系细胞上呈现的SRBC在体外激活形成抗体，而被另一亲本品系细胞上的SRBC激活的程度则低得多。无论抗原是呈现在巨噬细胞上还是由活化的T细胞呈递，都能观察到这种限制。同基因小鼠实验表明，这种限制由MHC基因座控制。F1 B细胞对任一亲本品系单倍型反应性的限制在骨髓中比在脾细胞群体中更容易诱导。在首次注射抗原3周后给经辐照的宿主再次注射抗原，这种限制可能会被打破；这可能反映了与供体F1型巨噬细胞相关的SRBC对Fl B细胞的“再教育”。正常未致敏的脾B细胞在与同基因活化T细胞联合时，对SRBC激活也表现出强烈的偏好。
