哇巴因对蛙皮电导率的影响。反对钠再循环的证据。

Effect of ouabain on electrical conductance of frog skins. Evidence against recycling of sodium.

作者信息

Corcia A, Lahav J, Caplan S R

出版信息

Biochim Biophys Acta. 1980 Feb 28;596(2):264-71. doi: 10.1016/0005-2736(80)90360-0.

Abstract

Ouabain (10(-4) M) added to the serosal solution of isolated frog skin not only stops the active transport of Na+ but causes a dramatic increase in the electric conductance of the skin. The effect also appears when ouabain is added to amiloride-pretreated skins, ruling out the possibility of a cellular effect. The similarity between the effect of ouabain on normal and amiloride-pretreated skins indicates that no appreciable recycling of Na+ across the basolateral membrane of frog skin takes place. The change of Na+ efflux after ouabain is added to amiloride-pretreated skins parallels the change of electric conductance, indicating that besides blocking the Na+ pump, ouabain affects the paracellular shunt pathway between the cells.

摘要

哇巴因（10⁻⁴ M）添加到离体蛙皮的浆膜溶液中，不仅会停止Na⁺的主动转运，还会使皮肤的电导显著增加。当哇巴因添加到用氨氯吡咪预处理过的皮肤时，也会出现这种效应，排除了细胞效应的可能性。哇巴因对正常皮肤和用氨氯吡咪预处理过的皮肤的作用相似，表明蛙皮基底外侧膜不存在明显的Na⁺再循环。将哇巴因添加到用氨氯吡咪预处理过的皮肤后，Na⁺外流的变化与电导的变化平行，表明除了阻断Na⁺泵外，哇巴因还影响细胞间的旁细胞分流途径。