用于微量凝集反应的立克次氏体抗原染色
Staining of rickettsial antigens for microagglutination reaction.
作者信息
Urvölgyi J
出版信息
J Hyg Epidemiol Microbiol Immunol. 1977;21(1):61-5.
PMID:69657
Abstract
Staining of antigens of R. prowazeki, R. canada and R. conori with hematoxylin-Harris for use in MAR was developed. Optimal results were obtained when rickettsial antigens were stained at 4 degrees C for 18 hours. Comparison of MAR with CFR has shown that MAR using stained rickettsial antigens is specific and as sensitive as CFR.
摘要
开发了用苏木精 - 哈里斯染色法对普氏立克次体、加拿大立克次体和康氏立克次体抗原进行染色以用于巨噬细胞抗体反应（MAR）的方法。当立克次体抗原在4℃下染色18小时时可获得最佳结果。巨噬细胞抗体反应（MAR）与补体结合反应（CFR）的比较表明，使用染色立克次体抗原的巨噬细胞抗体反应（MAR）具有特异性，且与补体结合反应（CFR）一样敏感。
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