通过固定一种酶诱导的两种酶之间的物理相互作用和活性偶联。
Physical interaction and activity coupling between two enzymes induced by immobilization of one.
作者信息
Tu S C, Hastings J W
出版信息
Proc Natl Acad Sci U S A. 1980 Jan;77(1):249-52. doi: 10.1073/pnas.77.1.249.
Abstract
Flavin reductase and bacterial luciferase are believed to be coupled in the in vivo light emitting reaction. In extracts, however, they are both soluble enzymes that exhibit little or no association. Immobilized luciferase, covalently attached to Sepharose, was found to bind the soluble reductase and to exhibit activity in the coupled reaction reaction with an enhanced efficiency of electron transfer.
摘要
黄素还原酶和细菌荧光素酶被认为在体内发光反应中是偶联的。然而，在提取物中，它们都是可溶性酶，几乎没有或没有结合。固定在琼脂糖上的荧光素酶被发现能结合可溶性还原酶，并在偶联反应中表现出活性，电子转移效率提高。
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本文引用的文献
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