Fink R, Grocki K, Lüttgau H C
Eur J Cell Biol. 1980 Apr;21(1):101-8.
The exhaustion of metabolically poisoned muscle fibres is accompanied by a one hundred-fold increase in potassium conductance. Under these conditions a normal membrane capacity was measured. Extracellular markers (lanthanum, ferritin) were traced in the transverse tubular system but not in the myoplasm or the sarcoplasmic reticulum. These results show that the structural integrity of surface and tubular membranes is fully maintained. The exhausted muscle can, therefore, be introduced as a suitable preparation for an analysis of the potassium channel, the alterations of ion concentrations in the restricted area of the T-system and other phenomena of physiological interest. Further experiments with extracellular markers dealt with the structure of the triads. Earlier measurements by other authors suggested a continuity between lumina of the T-system and the sarcoplasmic reticulum, at least after an exposure to hyperosmotic solutions. These results could not be confirmed.--In addition the distribution of fibre cross areas of sartorius muscle was analysed from cryosections.
代谢中毒的肌纤维耗竭时，钾电导增加百倍。在此条件下测得正常的膜电容。细胞外标记物（镧、铁蛋白）在横管系统中可被追踪到，但在肌浆或肌浆网中则不然。这些结果表明，表面膜和管膜的结构完整性得以充分维持。因此，衰竭肌肉可作为分析钾通道、T 系统受限区域离子浓度变化及其他生理相关现象的合适标本。用细胞外标记物进行的进一步实验涉及三联体的结构。其他作者早期的测量表明，至少在暴露于高渗溶液后，T 系统腔与肌浆网之间存在连续性。这些结果无法得到证实。此外，从冷冻切片分析了缝匠肌纤维横截面积的分布。
