Nitta T, Okumura S, Nakano M
Infect Immun. 1980 Apr;28(1):28-37. doi: 10.1128/iai.28.1.28-37.1980.
A butanol-extracted water-soluble adjuvant (Bu-WSA) obtained from Bacterionema matruchotii, a gram-positive oral bacterium, is a potent B-cell mitogen for murine lymphocytes in vitro. [(3)H]thymidine uptake of cultured spleen cells of BALB/c mice and nude athymic mice was greatly enhanced by the presence of Bu-WSA. Spleen cells which had been treated previously with rabbit anti-mouse thymocyte serum and guinea pig complement responded to Bu-WSA, whereas thymocytes and nylon wool column-filtered spleen cells were unresponsive to it. It was necessary for the adjuvant to be in the culture for at least 24 h in order to obtain significant lymphocyte activation. As macrophage-depleted spleen cells still responded to Bu-WSA, the proliferative response of lymphocytes seems to be independent of the presence of macrophages. The degree of response of spleen cells stimulated by a mixture of Bu-WSA and lipopolysaccharide (LPS) was very close to the sum of the responses stimulated by Bu-WSA and LPS individually. Spleen cells of LPS-injected mice, which were refractory to LPS, responded to Bu-WSA. On the other hand, the spleen cells of Bu-WSA-injected mice responded to LPS but not to Bu-WSA. Furthermore, the cells obtained from the spleens of mice which had been heavily cobalt 60 irradiated and bone marrow reconstituted 3 weeks in advance responded to LPS but not to Bu-WSA. Therefore, it appears that the cells responding to Bu-WSA were not identical to those responding to LPS.
从革兰氏阳性口腔细菌牙龈纤毛菌中提取的丁醇水溶性佐剂（Bu-WSA），在体外是一种对鼠淋巴细胞有强大作用的B细胞促有丝分裂原。Bu-WSA的存在极大地增强了BALB/c小鼠和无胸腺裸鼠培养脾细胞的[³H]胸腺嘧啶核苷摄取。预先用兔抗小鼠胸腺细胞血清和豚鼠补体处理过的脾细胞对Bu-WSA有反应，而胸腺细胞和经尼龙毛柱过滤的脾细胞对其无反应。佐剂必须在培养物中至少存在24小时才能获得显著的淋巴细胞活化。由于去除巨噬细胞的脾细胞仍对Bu-WSA有反应，淋巴细胞的增殖反应似乎与巨噬细胞的存在无关。Bu-WSA和脂多糖（LPS）混合物刺激的脾细胞反应程度非常接近Bu-WSA和LPS单独刺激的反应之和。注射LPS后对LPS不敏感的小鼠脾细胞对Bu-WSA有反应。另一方面，注射Bu-WSA的小鼠脾细胞对LPS有反应，但对Bu-WSA无反应。此外，从预先3周接受大剂量钴60照射并进行骨髓重建的小鼠脾脏中获得的细胞对LPS有反应，但对Bu-WSA无反应。因此，似乎对Bu-WSA有反应的细胞与对LPS有反应的细胞不同。
