Noma Y, Une M, Kihira K, Yasuda M, Kuramoto T, Hoshita T
J Lipid Res. 1980 Mar;21(3):339-46.
Gallbladder bile of the bullfrog, Rana cetesbeiana was found to contain a number of minor bile acids and bile alcohols in unconjugated forms. The following bile acids and bile alcohols were identified by combined gas-liquid chromatography-mass spectrometry: cholic acid, allocholic acid, 3 alpha,7 alpha,12 alpha-trihydroxy-5 alpha-cholestan-26-oic acid, 3 alpha,7 alpha,12 alpha-trihydroxy-24-methyl-5 beta-cholestan-26-oic acid, 3 alpha,7 alpha,12 alpha,24-tetrahydroxy-5 alpha-cholestan-26-oic acid, 3 alpha,7 alpha,12 alpha,24-tetrahydroxy-5 beta-cholestan-26-oic acid, 3 alpha,7 alpha,12 alpha,26-tetrahydroxy-5 alpha-cholestan-27-oic acid, 3 alpha,7 alpha,12 alpha,26-tetrahydroxy-5 beta-cholestan-27-oic acid, 3 alpha,7 alpha,12 alpha-trihydroxy-27-nor-5 alpha-cholestan-24-one, and 3 alpha,7 alpha,12 alpha-trihydroxy-27-nor-5 beta-cholestan-24-one. These minor constituents of the bile may represent intermediates in the biosynthetic pathway of bile acids and bile alcohols in the bullfrog.
人们发现，美国牛蛙（Rana cetesbeiana）胆囊胆汁中含有多种未结合形式的次要胆汁酸和胆汁醇。通过气液色谱-质谱联用技术鉴定出了以下胆汁酸和胆汁醇：胆酸、别胆酸、3α,7α,12α-三羟基-5α-胆甾烷-26-酸、3α,7α,12α-三羟基-24-甲基-5β-胆甾烷-26-酸、3α,7α,12α,24-四羟基-5α-胆甾烷-26-酸、3α,7α,12α,24-四羟基-5β-胆甾烷-26-酸、3α,7α,12α,26-四羟基-5α-胆甾烷-27-酸、3α,7α,12α,26-四羟基-5β-胆甾烷-27-酸、3α,7α,12α-三羟基-27-降-5α-胆甾烷-24-酮和3α,7α,12α-三羟基-27-降-5β-胆甾烷-24-酮。胆汁中的这些次要成分可能代表了牛蛙胆汁酸和胆汁醇生物合成途径中的中间体。
