急性白血病患儿及其家庭成员中白血病相关抗原的白细胞迁移抑制

Leukocyte migration inhibition of leukemia-associated antigen in children with acute leukemia and their family members.

作者信息

Hann H W, London W T, Evans A E, Hann R S

出版信息

Cancer. 1980 Jul 15;46(2):293-8. doi: 10.1002/1097-0142(19800715)46:2<293::aid-cncr2820460213>3.0.co;2-2.

DOI:10.1002/1097-0142(19800715)46:2<293::aid-cncr2820460213>3.0.co;2-2

Abstract

The leukocyte migration inhibition assay has been widely used as an in vitro test cell mediated immunity to various substances. Using a leukemic cell line (RPMI 8402) as the source of antigen, we assayed leukocyte migration inhibition in seven children with acute lymphocytic leukemic (ALL), their family members (14 patients and 13 siblings), and 17 healthy adult controls. In six families, the patients showed stronger inhibition than their family members or controls. The patients differed significantly from their family members (P = 0.003) and all other persons without leukemia (P = 0.02). These results suggest that patients with ALL have developed a specific cellular immune reactivity to an antigen or antigens on leukemic cells, while their family members have not.

摘要

白细胞迁移抑制试验已被广泛用作一种体外测试细胞介导的针对各种物质的免疫反应。我们以一种白血病细胞系（RPMI 8402）作为抗原来源，检测了7例急性淋巴细胞白血病（ALL）患儿、他们的家庭成员（14名患者和13名兄弟姐妹）以及17名健康成年对照者的白细胞迁移抑制情况。在6个家庭中，患儿表现出比其家庭成员或对照者更强的抑制作用。患儿与他们的家庭成员之间差异显著（P = 0.003），与所有其他无白血病的人之间差异也显著（P = 0.02）。这些结果表明，ALL患儿已对白血病细胞上的一种或多种抗原产生了特异性细胞免疫反应，而他们的家庭成员则没有。