Vaca G, Sanchez-Corona J, Olivares N, Medina C, Ibarra B, Cantu J M
Ann Genet. 1980;23(2):126-8.
A simple fluorescent test for the activity of blood uridine diphosphate galactose 4-epimerase which converts uridine diphosphate galactose to uridine diphosphate glucose is described. The enzyme activity is visually estimated by to reduction of NAD+ (non fluorescent) to NADH (fluorescent) in a coupled reaction with uridine diphosphate glucose dehydrogenase. The appearance of fluorescence indicates that epimerase activity is present in the sample. The usefulness of this test in newborn screening programs for inborn errors of galactose metabolism is stressed.
本文描述了一种用于检测血液中尿苷二磷酸半乳糖4-表异构酶活性的简单荧光试验，该酶可将尿苷二磷酸半乳糖转化为尿苷二磷酸葡萄糖。通过在与尿苷二磷酸葡萄糖脱氢酶的偶联反应中，将NAD +（无荧光）还原为NADH（有荧光），直观地估计酶活性。荧光的出现表明样品中存在表异构酶活性。强调了该试验在新生儿半乳糖代谢先天性缺陷筛查项目中的实用性。
