Hirata K, Nada O
Histochemistry. 1980;67(1):65-71. doi: 10.1007/BF00490088.
Sequential changes in the monoamine-containing cell (MC cell) of the developing frog tongue has been studied by fluorescence histochemistry using uptake of 5,6-dihydroxytryptamine. At st. 16, a few yellow fluorescent cells, here called MC cells, appeared in random order at the uppermost layer of the dorsal epithelium. They were round or elliptical in shape. At st. 18 the MC cells, greatly transformed, were found at the periphery of the sensory disc primordium which first appears during this stage. The MC cell was made up of three parts: perikaryon, process and terminal portion. The perikaryon was located at the upper half of the epithelium and from it a single process stretched vertically toward the basal lamina, above which the dilated terminal portion was found. Thereafter the perikaryon gradually moved toward the basal layer while remaining at the periphery of the disc primordium. Meanwhile the terminal portion moved over the basal lamina toward the center of the disc primordium. At st. 22, the whole of the MC cell lay flat above the basal lamina. The perikaryon was localized at the periphery of the sensory disc and from there the process stretched toward the center. Thus, the morphology of MC cells resembled the adult state, except for smaller size. MC cells were never observed in the subepithelial connective tissue in the present study. This seems to suggest that the MC cell of the frog fungiform papilla is of epithelial origin.
利用5,6 - 二羟基色胺摄取的荧光组织化学方法，对发育中的蛙舌含单胺细胞（MC细胞）的顺序变化进行了研究。在第16期，一些黄色荧光细胞（此处称为MC细胞）随机出现在背侧上皮的最上层。它们呈圆形或椭圆形。在第18期，MC细胞发生了巨大转变，位于在此阶段首次出现的感觉盘原基的周边。MC细胞由三部分组成：核周体、突起和终末部分。核周体位于上皮的上半部分，从它伸出一个单一的突起垂直朝向基底层，在基底层上方发现了扩张的终末部分。此后，核周体逐渐向基底层移动，同时仍位于盘原基的周边。与此同时，终末部分越过基底层向盘原基的中心移动。在第22期，整个MC细胞平躺在基底层上方。核周体位于感觉盘的周边，从那里突起向中心延伸。因此，MC细胞的形态除了尺寸较小外，类似于成年状态。在本研究中，上皮下结缔组织中从未观察到MC细胞。这似乎表明蛙菌状乳头的MC细胞起源于上皮。
