Edelmann L
Histochemistry. 1980;67(3):233-42. doi: 10.1007/BF00692757.
Normal frog sartorius muscles and muscles in which a major portion of the intracellular K+ was reversibly replaced by Rb+ or Cs+ were frozen, freeze-dried and embedded without chemical fixation or staining. Dry-cut sections of these preparations reveal striation patterns with higher contrast than those of wet-cut sections of the same preparation. The results suggest that in the living state the alkali metal ions are mainly localized in the A bands and Z lines of myofibrils. This idea is confirmed by a new autoradiographic technique by means of which the distribution of Rb+ and Cs+ in frozen-hydrated single muscle fibers has been investigated. The findings support the association-induction hypothesis according to which most cell K+ and other alkali-metal ions are not free in cell water but are adsorbed to beta- and gamma-carboxyl groups of cell proteins.
将正常青蛙的缝匠肌以及细胞内大部分钾离子被铷离子或铯离子可逆替代的肌肉进行冷冻、冻干并包埋，无需化学固定或染色。这些标本的干切片显示出的条纹图案比同一标本湿切片的对比度更高。结果表明，在活体状态下，碱金属离子主要定位于肌原纤维的A带和Z线。这一观点通过一种新的放射自显影技术得到了证实，利用该技术研究了铷离子和铯离子在冷冻水合单根肌纤维中的分布。这些发现支持了缔合-诱导假说，根据该假说，大多数细胞内的钾离子和其他碱金属离子并非游离于细胞水中，而是吸附在细胞蛋白质的β-和γ-羧基上。
