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球形红假单胞菌外膜的蛋白质组成

Protein composition of Rhodopseudomonas sphaeroides outer membrane.

作者信息

Baumgardner D, Deal C, Kaplan S

出版信息

J Bacteriol. 1980 Jul;143(1):265-73. doi: 10.1128/jb.143.1.265-273.1980.

DOI:10.1128/jb.143.1.265-273.1980
PMID:6967483
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC294223/
Abstract

The outer membrane polypeptide profile of Rhodopseudmonas sphaeroides was characterized. Solubilization of the outer membrane at 75 or 100 degrees C as opposed to room temperature resulted in the dissociation of 75-, 72-, and 68-kilodalton (kdal) polypeptide aggregates into 29-, 26.5-, and 21.5-kdal polypeptides, respectively, and a shared 47-kdal subunit. Similarly, an 88.5-kdal polypeptide dissociates into a 45-kdal monomeric form, and the electrophoretic mobility of a 58.5-kdal polypeptide was altered to 83 kdal. Lysozyme treatment of outer membrane fractions altered the 21.5-kdal polypeptide mobility to 23 kdal. The presence of lipid in both the 47-kdal polypeptide and an 8- to 10-kdal polypeptide was demonstrated by lipid staining and [14C]acetate incorporation. The lipid component of the 47-kdal polypeptide was neither lipopolysaccharide nor phospholipid. The 8- to 10-kdal polypeptide may be the equivalent of the Braun lipoprotein. Outer membrane fractions isolated from R. sphaeroides-specific phage RS1-resistant mutants were deficient in several of the high-molecular-weight aggregates involving the 47-kdal polypeptide.

摘要

对球形红假单胞菌的外膜多肽谱进行了表征。与室温相比，在75或100摄氏度下对外膜进行溶解，导致75、72和68千道尔顿（kdal）的多肽聚集体分别解离为29、26.5和21.5 kdal的多肽，以及一个共享的47 kdal亚基。同样，一个88.5 kdal的多肽解离为45 kdal的单体形式，并且一个58.5 kdal多肽的电泳迁移率改变为83 kdal。对外膜组分进行溶菌酶处理后，21.5 kdal多肽的迁移率改变为23 kdal。通过脂质染色和[14C]乙酸掺入证明了47 kdal多肽和8至10 kdal多肽中均存在脂质。47 kdal多肽的脂质成分既不是脂多糖也不是磷脂。8至10 kdal多肽可能等同于布劳恩脂蛋白。从球形红假单胞菌特异性噬菌体RS1抗性突变体中分离的外膜组分在涉及47 kdal多肽的几种高分子量聚集体中存在缺陷。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/902a/294223/b515ea9acf8c/jbacter00568-0286-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/902a/294223/9fb2efe7565c/jbacter00568-0283-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/902a/294223/b27a57b43d57/jbacter00568-0284-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/902a/294223/fd075270e3c2/jbacter00568-0285-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/902a/294223/a928aa595f27/jbacter00568-0285-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/902a/294223/8c03d4fba4ae/jbacter00568-0286-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/902a/294223/b515ea9acf8c/jbacter00568-0286-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/902a/294223/9fb2efe7565c/jbacter00568-0283-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/902a/294223/b27a57b43d57/jbacter00568-0284-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/902a/294223/fd075270e3c2/jbacter00568-0285-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/902a/294223/a928aa595f27/jbacter00568-0285-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/902a/294223/8c03d4fba4ae/jbacter00568-0286-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/902a/294223/b515ea9acf8c/jbacter00568-0286-b.jpg

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