Shurin P A, Pelton S I, Tager I B, Kasper D L
J Pediatr. 1980 Sep;97(3):364-9. doi: 10.1016/s0022-3476(80)80182-x.
We have developed a bactericidal antibody assay to determine the role of circulating antibody to nontypable strains of Haemophilus influenzae in children with otitis media. Antibody infecting strain was present in the acute sera of 2/20 children with otitis media caused by H. influenzae NT; 15/18 of these patients had covalescent specimens with titers greater than or equal to 1:2 (X2 = 13.0, P < 0.001). The acute sera of 95 children with otitis media caused by various organisms were screened for bactericidal activity against a randomly selected H. influenzae NT stain. Antibody was present in 0/28 acute sera of patients whose infection was caused by H. influenzae NT and in 18/67 (26.9% of those whose middleear exudates were sterile or contained other bacterial species (X2 = 9.95, P < 0.01). A bactericidal antibody response generally follows infection of the middlw ear with H. influenzae NT. The absence of antibody to a single strain in the sera of some children appears to be associated with susceptibility to this infection.
我们开发了一种杀菌抗体检测方法，以确定循环抗体在患中耳炎儿童中对不可分型流感嗜血杆菌菌株的作用。在20例由非典型流感嗜血杆菌引起中耳炎的儿童中，有2例的急性期血清中存在感染菌株抗体；这些患者中有15/18例恢复期标本的滴度大于或等于1:2（X2 = 13.0，P < 0.001）。对95例由各种病原体引起中耳炎的儿童急性期血清进行了针对随机选择的非典型流感嗜血杆菌菌株的杀菌活性筛查。在感染由非典型流感嗜血杆菌引起的患者的28例急性期血清中，有0例存在抗体，而在67例中耳渗出液无菌或含有其他细菌种类的患者中，有18例（占26.9%）存在抗体（X2 = 9.95，P < 0.01）。杀菌抗体反应通常在中耳受到非典型流感嗜血杆菌感染后出现。一些儿童血清中缺乏针对单一菌株的抗体似乎与对这种感染的易感性有关。
