血清抗体对三种不可分型流感嗜血杆菌外膜蛋白的反应在易患和不易患中耳炎的儿童急性中耳炎和鼻咽定植期间。
Serum antibody response to three non-typeable Haemophilus influenzae outer membrane proteins during acute otitis media and nasopharyngeal colonization in otitis prone and non-otitis prone children.
机构信息
Rochester General Hospital Research Institute, 1425 Portland Avenue, Rochester, NY 14621, USA.
出版信息
Vaccine. 2011 Jan 29;29(5):1023-8. doi: 10.1016/j.vaccine.2010.11.055. Epub 2010 Dec 1.
Non-typeable Haemophilus influenzae (NTHi) is the most common bacteria responsible for episodic acute otitis media (AOM; non-otitis prone), recurrent AOM (rAOM; otitis prone) and AOM treatment failure (AOMTF) in children. In this 3.5 years of prospective study, we measured the serum antibody response to outer membrane proteins D, P6 and OMP26 of NTHi in children with AOM (n=26), rAOM (n=32), AOMTF (n=27). The geometric mean titers (GMTs) of IgG at their acute AOM visit against Protein D in otitis prone children were significantly lower compared to AOMTF (p value<0.01) and non-otitis prone (p value<0.03) children; otitis prone children had significantly lower IgG levels to P6 compared to AOMTF children (p value<0.02); otitis prone children had significantly lower IgG levels to OMP26 compared to AOMTF children (p value<0.04). Comparing acute to convalescent titers after AOM, otitis prone and AOMTF children had no significant change in total IgG against all the three proteins, while non-otitis prone children had significant increases to Protein D. Anti-protein D, P6 and OMP26 antibody levels measured longitudinally during NP colonization between age 6 and 24 months in 10 otitis prone children and 150 non-otitis prone children showed <2-fold increases over time in otitis prone children compared to >4 fold increases in the non-otitis prone children (p value<0.001). We conclude that otitis prone children mount less of an IgG serum antibody response toward Protein D, P6 and OMP26 after AOM which may account for recurrent infections. The data on acute sera of otitis prone vs non-otitis prone children and the acute-to-convalescence response in non-otitis prone children point to a possible link of anti-PD to protection. Moreover, the data suggest that otitis prone children should be evaluated for their responses to Protein D, P6 and OMP26 vaccine antigens of NTHi.
无乳链球菌（NTHi）是引起儿童偶发性急性中耳炎（AOM；非易感性）、复发性 AOM（rAOM；易感性）和 AOM 治疗失败（AOMTF）的最常见细菌。在这项为期 3.5 年的前瞻性研究中，我们测量了血清抗体对 NTHi 外膜蛋白 D、P6 和 OMP26 的反应，这些儿童患有 AOM（n=26）、rAOM（n=32）和 AOMTF（n=27）。易感性儿童在急性 AOM 就诊时针对蛋白 D 的 IgG 几何平均滴度（GMT）明显低于 AOMTF（p 值<0.01）和非易感性儿童（p 值<0.03）；与 AOMTF 儿童相比，易感性儿童的 P6 IgG 水平显著降低（p 值<0.02）；与 AOMTF 儿童相比，易感性儿童的 OMP26 IgG 水平显著降低（p 值<0.04）。比较急性和 AOM 后恢复期的滴度，易感性和 AOMTF 儿童针对所有三种蛋白的总 IgG 没有明显变化，而非易感性儿童的蛋白 D 显著增加。在 10 名易感性儿童和 150 名非易感性儿童中，在 6 至 24 个月期间 NP 定植期间纵向测量抗蛋白 D、P6 和 OMP26 抗体水平，易感性儿童的抗体水平随时间的变化增加<2 倍，而非易感性儿童的增加>4 倍（p 值<0.001）。我们得出结论，与非易感性儿童相比，易感性儿童在 AOM 后针对蛋白 D、P6 和 OMP26 的 IgG 血清抗体反应较少，这可能导致反复感染。易感性与非易感性儿童急性血清的比较数据以及非易感性儿童急性到恢复期的反应表明抗 PD 可能与保护有关。此外，数据表明，应评估易感性儿童对 NTHi 的蛋白 D、P6 和 OMP26 疫苗抗原的反应。
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