Mookerjee B K, Ferber E, Ernst M, Sharon N, Fischer H
Immunol Commun. 1980;9(7):653-76. doi: 10.3109/08820138009066016.
Rat thymocytes respond to exposure to plant lectins by a burst of oxidant generation as detected by chemiluminescence (CL) (CL) in presence of luminol. All lectins tested were capable of evoking CL as long as sufficiently high concentration was used. There was no correlation between the capacity of a given lectin to evoke CL on one hand and its ability to cause mitogenic transformation or thymocyte agglutination on the other. During the first few minutes following exposure to Con A, cells in the thymocyte pool become committed to CL but once commitment is induced, removal of cell bound ligand by addition of excess methyl-alpha-D-mannoside does not significantly alter the response. Succinylated Con A is as effective as native Con A at lower concentrations but is much more effective at higher concentrations in evoking CL. Thymocyte CL can occur in absence of extracellular calcium but this calcium-independent CL is reduced by the addition of magnesium. In presence of calcium, CL response is greatly augmented and the calcium dependent response is not inhibited in presence of magnesium. The response is relatively radiosensitive. Thymocyte CL response is inhibitable by catalase and scavengers of oxidative radicals but is relatively resistant to the effects of superoxide dismutase, requiring high concentrations of significant effect.
通过在鲁米诺存在下的化学发光（CL）检测发现，大鼠胸腺细胞暴露于植物凝集素时会产生一阵氧化剂。只要使用足够高的浓度，所有测试的凝集素都能够引发CL。一方面，给定凝集素引发CL的能力与其引起有丝分裂转化或胸腺细胞凝集的能力之间没有相关性。在暴露于刀豆球蛋白A后的最初几分钟内，胸腺细胞库中的细胞开始产生CL，但一旦诱导产生这种反应，通过添加过量的甲基-α-D-甘露糖苷去除细胞结合的配体并不会显著改变反应。琥珀酰化刀豆球蛋白A在较低浓度下与天然刀豆球蛋白A一样有效，但在较高浓度下引发CL时效果要好得多。胸腺细胞CL可以在没有细胞外钙的情况下发生，但这种不依赖钙的CL会因添加镁而降低。在有钙的情况下，CL反应会大大增强，并且在有镁的情况下，钙依赖性反应不会受到抑制。该反应对辐射相对敏感。胸腺细胞CL反应可被过氧化氢酶和氧化自由基清除剂抑制，但对超氧化物歧化酶的作用相对抗性，需要高浓度才有显著效果。
