Lassalles J P, Hartmann A, Thellier M
J Membr Biol. 1980 Sep 30;56(2):107-19. doi: 10.1007/BF01875962.
When a frog skin is used to separate two compartments, and lithium is added to the external medium, transmembrane electric potential oscillations frequently occur. When no external current is imposed, sustained oscillations, with a period of about 10 min, are maintained for several hours. An oscillation of the Na+ influx accompanies the electric oscillation, though the two oscillations are out of phase to a greater or less extent. Theophyllin promotes a significant decrease in the mean electric potential of the skin, but it does not affect very much the characteristics of the oscillation. Important factors influencing the oscillation are temperature, permeability of the external membrane to lithium, and potassium concentration in the internal medium. No correlation can be detected between oscillation characteristics and skin area. This suggests that the oscillation is of a local nature, possibly originating at the cellular level. Occurrence of macroscopic oscillations implies coupling between local oscillators. Coupling between two epithelia has been studied under diverse conditions. The coupling is of an electrical nature: by varying the value of the coupling resistance, it is possible to control synchronization of the oscillations.
当用蛙皮分隔两个腔室，并向外部介质中添加锂时，经常会出现跨膜电位振荡。在不施加外部电流的情况下，持续振荡（周期约为10分钟）会持续数小时。尽管这两种振荡在或多或少的程度上不同步，但Na⁺内流的振荡伴随着电振荡。茶碱可使皮肤的平均电位显著降低，但对振荡特性的影响不大。影响振荡的重要因素包括温度、外膜对锂的通透性以及内部介质中的钾浓度。未检测到振荡特性与皮肤面积之间的相关性。这表明振荡具有局部性质，可能起源于细胞水平。宏观振荡的出现意味着局部振荡器之间存在耦合。已经在不同条件下研究了两个上皮之间的耦合。这种耦合是电性质的：通过改变耦合电阻的值，可以控制振荡的同步。
