Poulsen P B, Claësson M H
Differentiation. 1980;17(2):77-84. doi: 10.1111/j.1432-0436.1980.tb01083.x.
B lymphocyte colony development in agar culture was studied using an electron microscope, and more than 3,000 colony cells were identified and photographed. In early cultures (day 4) lymphoblasts dominated the colonies. From day 5 onwards plasmablasts and small lymphocytes were present in colonies. From day 6 onward mature plasma cells were observed in increasing numbers. On day 9 culture the colonies started to degenerate and on day 10 of culture approximately 70% of the colony consisted of pyknotic and degenerating cells. Topographically, the degenerating cells were concentrated in the center of the colony whereas proliferation took place in the periphery. Colony growth occurred in an exponential fashion, the number of viable colony cells being maximal on day 8 of culture (400-600 cells/colony). At this time the frequencies of the four B cell categories were: lymphoblasts 72%, plasmablasts 20%, plasma cells 6%, and small lymphocytes 2%. Recloning experiments showed that dispersed colony cells were capable of forming only small cell clusters. It is concluded that B lymphocyte colony formation reflects a series of B cell developmental stages including the formation of the end cell categories o this lymphocyte lineage.
利用电子显微镜研究了琼脂培养中B淋巴细胞集落的发育情况，鉴定并拍摄了3000多个集落细胞。在培养早期（第4天），淋巴母细胞在集落中占主导地位。从第5天起，成浆细胞和小淋巴细胞出现在集落中。从第6天起，观察到成熟浆细胞数量不断增加。在培养第9天时，集落开始退化，在培养第10天时，约70%的集落由固缩和退化细胞组成。从地形学上看，退化细胞集中在集落中心，而增殖发生在外围。集落生长呈指数方式，培养第8天时活集落细胞数量最多（400 - 600个细胞/集落）。此时，四类B细胞的频率分别为：淋巴母细胞72%，成浆细胞20%，浆细胞6%，小淋巴细胞2%。再克隆实验表明，分散的集落细胞只能形成小细胞簇。结论是，B淋巴细胞集落形成反映了一系列B细胞发育阶段，包括该淋巴细胞谱系终末细胞类别的形成。
