Lewis D E, Giorgi J V, Warner N L
Nature. 1981 Jan 22;289(5795):298-300. doi: 10.1038/289298a0.
The study of spontaneous autoimmunity in mouse models affords an opportunity to determine the cellular basis of the immune dysregulation observed in this disease. Recently, a new mouse strain, MRL/Mp-Ipr/Ipr (MRL/l) has been developed which carries an autosomal recessive gene (lpr) that results in massive lymph node enlargement concomitant with the development of several autoantibodies. The interest in this strain lies in the possibility that the defect in T-cell regulation of the immune response is manifested at a different level from that in the NZB mouse. It has been reported that the proliferating population of lymphoid cells in the nodes of these mice are T cells, but that many of them are devoid of Lyt surface antigens. We have accordingly initiated several lines of research with these mice, including quantitative flow cytometry characterization of Lyt antigen expression of cells in the lymph nodes of the mice. In an approach to isolate and study the properties of these cells, we have also established continuous cell lines from the lymph node cells of MRL/l mice, using techniques similar to those used to establish continuous lines of antigen-activated cytotoxic T cells and helper T-cell populations.
对小鼠模型中自发自身免疫的研究为确定该疾病中观察到的免疫失调的细胞基础提供了一个机会。最近，一种新的小鼠品系，即MRL/Mp-Ipr/Ipr（MRL/l）已被培育出来，它携带一个常染色体隐性基因（lpr），该基因导致大量淋巴结肿大，并伴有多种自身抗体的产生。对该品系的兴趣在于，免疫反应的T细胞调节缺陷可能在与NZB小鼠不同的水平上表现出来。据报道，这些小鼠淋巴结中增殖的淋巴细胞群体是T细胞，但其中许多缺乏Lyt表面抗原。因此，我们用这些小鼠开展了多项研究，包括对小鼠淋巴结中细胞Lyt抗原表达进行定量流式细胞术分析。为了分离并研究这些细胞的特性，我们还利用类似于建立抗原激活的细胞毒性T细胞和辅助性T细胞群体连续系所使用的技术，从MRL/l小鼠的淋巴结细胞中建立了连续细胞系。
