Winton E F, Kinkade J M, Vogler W R, Parker M B, Barnes K C
Blood. 1981 Mar;57(3):574-8.
Human lactoferrin (LF) has been reported to inhibit in vitro granulopoiesis by means of decreasing colony-stimulating activity production by monocytes. We performed a series of experiments to determine if the reported experimental results could be replicated using highly purified murine LF and murine target cells. Three different types of experiments were performed. (1) Medium was conditioned by lung, femoral shaft, and adherent peritoneal cells in the presence and absence of LF, and the granulopoietic stimulating activity in the conditioned media was assayed by means of a 7-day agar colony assay and a 3-day liquid slide chamber assay, which quantitates 3H-TdR incorporation into DNA. (2) In cultures stimulated by an underlayer of adherent peritoneal cells, marrow cell colony formation in agar was determined after 7 days of culture in the presence or absence of LF. (3) LF was added to 3-day liquid marrow cell cultures that had been stimulated by lung or femoral shaft conditioned media. In all experimental situations, highly purified, iron-saturated LF in concentrations up to 10(-7) M had no effect on in vitro granulopoiesis. These results do not support LF's reputed regulatory role in granulopoiesis.
据报道，人乳铁蛋白（LF）可通过降低单核细胞产生集落刺激活性来抑制体外粒细胞生成。我们进行了一系列实验，以确定使用高度纯化的小鼠LF和小鼠靶细胞是否能够重复报道的实验结果。进行了三种不同类型的实验。（1）在有或无LF的情况下，用肺、股骨干和贴壁腹膜细胞对培养基进行预处理，然后通过7天琼脂集落测定法和3天液体玻片小室测定法（该方法通过定量3H-TdR掺入DNA来检测）检测预处理培养基中的粒细胞生成刺激活性。（2）在由贴壁腹膜细胞下层刺激的培养物中，在有或无LF的情况下培养7天后，测定琼脂中骨髓细胞集落的形成情况。（3）将LF添加到由肺或股骨干预处理培养基刺激的3天液体骨髓细胞培养物中。在所有实验情况下，浓度高达10^(-7) M的高度纯化、铁饱和的LF对体外粒细胞生成均无影响。这些结果不支持LF在粒细胞生成中所声称的调节作用。
