Vos J G, Roholl P J, Leene W
Cell Tissue Res. 1980;213(2):221-35. doi: 10.1007/BF00234783.
Density separation of purified peripheral blood leucocytes from T-cell depleted rabbits on a linear Ficoll-metrizoate gradient has been applied to obtain different leucocyte fractions. Two lymphocyte fractions separated on density seem to have different characteristics, both morphologically and immunologically. In this study these two fractions have been characterized ultrastructurally by using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and a relationship has been established between the surface architecture (SEM), the cell size (SEM/TEM) and surface-Ig/C3-receptors (LM, light microscopy). Finally three types of lymphocytes have been described in the two lymphocyte fractions separated on density. Morphometric information such as cell size, cell shape, eu-/heterochromatin ratio in the nucleus and the nucleus-/cell ratio have been correlated to the stage of activation of the B lymphocyte in a representative density separation.
利用线性聚蔗糖-泛影酸盐梯度对T细胞耗竭兔的纯化外周血白细胞进行密度分离，以获得不同的白细胞组分。在密度上分离出的两个淋巴细胞组分在形态学和免疫学上似乎具有不同的特征。在本研究中，通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）对这两个组分进行了超微结构表征，并建立了表面结构（SEM）、细胞大小（SEM/TEM）与表面免疫球蛋白/C3受体（光镜，LM）之间的关系。最后，在密度分离出的两个淋巴细胞组分中描述了三种淋巴细胞类型。在一次代表性的密度分离中，诸如细胞大小、细胞形状、细胞核中常染色质/异染色质比例以及细胞核/细胞比例等形态测量信息已与B淋巴细胞的活化阶段相关联。
