Van Wauwe J, Goossens J
Immunology. 1981 Jan;42(1):157-64.
The complement-mediated lysis of human lymphocytes by three monoclonal anti-human T-cell antibodies OKT3.PAN, OKT4.INd and OKT8.SUP was studied. The percentages of Ficoll-Hypaque-isolated mononuclear cells lysed by these antibodies were respectively: 65% for OKT3.PAN, 39% for OKT4.IND and 20% for OKT8.SUP. Optimal lymphocytotoxic reactions were noticed when unabsorbed rabbit serum was used as the source of complement (C). Addition of heat-inactivated human, mouse and newborn calf sera but not of foetal calf serum inhibited the lytic activity of the antibodies. Treatment of peripheral mononuclear blood cells with OKT3.PAN and C abrogated their mitotic response to PHA and Con-A. Sheep erythrocyte rosetting lymphocytes (E+ cells) treated with OKT4.IND or OKT8.SUP and C exhibited no marked changes in responsiveness to PHA, Con-A or allogeneic non-T cells. However, only E+ cells enriched with OKT4.IND-reactive cells responded to purified protein derivative, proliferated in the autologous mixed lymphocyte reaction and were highly sensitive to hydrocortisone suppression when stimulated by PHA. Our data indicate that these monoclonal antibodies can be regarded as invaluable tools for enumeration, characterization and functional assessment of human T cells and their subclasses.
研究了三种单克隆抗人T细胞抗体OKT3.PAN、OKT4.INd和OKT8.SUP介导的人淋巴细胞补体溶解作用。这些抗体对经Ficoll-Hypaque分离的单核细胞的裂解百分比分别为:OKT3.PAN为65%,OKT4.IND为39%,OKT8.SUP为20%。当使用未吸收的兔血清作为补体(C)来源时,观察到最佳的淋巴细胞毒性反应。添加热灭活的人、小鼠和新生小牛血清而非胎牛血清可抑制抗体的裂解活性。用OKT3.PAN和C处理外周血单核细胞可消除它们对PHA和Con-A的有丝分裂反应。用OKT4.IND或OKT8.SUP和C处理的绵羊红细胞玫瑰花结淋巴细胞(E+细胞)对PHA、Con-A或同种异体非T细胞的反应性未显示明显变化。然而,只有富含OKT4.IND反应性细胞的E+细胞对纯化蛋白衍生物有反应,在自体混合淋巴细胞反应中增殖,并且在受到PHA刺激时对氢化可的松抑制高度敏感。我们的数据表明,这些单克隆抗体可被视为用于人类T细胞及其亚类的计数、表征和功能评估的宝贵工具。