Sawada U, Adler S S
Exp Hematol. 1980 Jul;8(6):702-8.
To evaluate the effects of thymocytes from anemic W/WV mice on hemopoiesis in vitro, we co-cultured thymocytes from W/WV mice or from their non-anemic +/+ littermates with W/WV or +/+ marrow cells in semi-solid systems designed to foster colonial growth from neutrophil/macrophage (CFUNM), early erythroid (BFUE), late erythroid (CFUE) precursors. The marrow cell to thymocyte ratios in the cultures were 1:3 and 1:20 (1:175 for CFUNM). Untreated or anti-theta serum (ATS) or mock (control) AT treated marrow cells were cultured with the thymocytes. We found that marrow from +/+ and W/WV mice contain equivalent concentrations of CFUNM but W/WV marrow has a lower concentration of BFUE than +/+ marrow. Neither +/+ nor W/WV thymoctyes, in the concentrations used, effected any increase in the numbers of CFUNM or CFUE colonies formed from untampered or antiserum treated marrow cells. In two groups, thymocytes effected a slight increase in the numbers of bursts (BFUE colonies) formed from marrow cell suspensions and in both of these groups W/WV thymocytes were as effective as +/+ thymocytes. Thus, under the conditions studied, W/WV thymocytes were not more inhibitory or less stimulatory for in vitro hemopoiesis than were normal thymocytes.
为了评估贫血的W/WV小鼠的胸腺细胞对体外造血的影响，我们在旨在促进中性粒细胞/巨噬细胞（CFU-NM）、早期红系（BFU-E）、晚期红系（CFU-E）前体细胞集落生长的半固体系统中，将W/WV小鼠或其非贫血的+/+同窝小鼠的胸腺细胞与W/WV或+/+骨髓细胞共培养。培养物中骨髓细胞与胸腺细胞的比例为1:3和1:20（CFU-NM为1:175）。未经处理或用抗θ血清（ATS）或模拟（对照）抗胸腺细胞血清（AT）处理的骨髓细胞与胸腺细胞一起培养。我们发现，+/+和W/WV小鼠的骨髓中CFU-NM的浓度相当，但W/WV骨髓中BFU-E的浓度低于+/+骨髓。在所使用的浓度下，+/+和W/WV胸腺细胞均未使未处理或抗血清处理的骨髓细胞形成的CFU-NM或CFU-E集落数量增加。在两组中，胸腺细胞使骨髓细胞悬液形成的爆式集落（BFU-E集落）数量略有增加，并且在这两组中，W/WV胸腺细胞与+/+胸腺细胞一样有效。因此，在所研究的条件下，W/WV胸腺细胞对体外造血的抑制作用并不比正常胸腺细胞更强，刺激作用也不比正常胸腺细胞更弱。
