Fauci A S, Moutsopoulos H M
Arthritis Rheum. 1981 Apr;24(4):577-83. doi: 10.1002/art.1780240402.
Numbers of B cells spontaneously secreting Ig (IgG, IgA, and IgM) were determined by a plaque-forming cell (PFC) assay simultaneously in the peripheral blood and bone marrow of normal individuals, patients with systemic lupus erythematosus (SLE), and patients with primary Sjögren's syndrome. Normal individuals had 382 (+/- 89) PFC per 10(6) mononuclear cells in peripheral blood. Patients with either active or inactive Sjögren's syndrome had normal numbers of spontaneous Ig-secreting cells in peripheral blood (P greater than 0.2). Conversely, patients with inactive as well as active SLE had markedly increased spontaneous PFC (P less than 0.05 and P less than 0.001, respectively). Patients with active SLE had significantly greater PFC than patients with inactive SLE; 3,984 (+/- 960) versus 1,605 (+/- 527) PFC per 10(6) mononuclear cells (P less than 0.05). The lack of increased numbers of activated B cells in the blood of patients with Sjögren's syndrome was not explained by a preferential sequestration of activated B cells in the bone marrow. However, of particular interest was the finding that the bone marrow served not only as a major source of virgin B cells but as a lymphoid organ of either in situ activation of B cells or sequestration for activated B cells. Normal individuals had approximately a 20-fold relative increase of activated B cells per 10(6) mononuclear cells in the bone marrow compared to peripheral blood, while patients with inactive and active SLE both had a 35-fold relative increase in activated B cells in bone marrow compared to peripheral blood. The potential relevance of circulating activated B cells and their sequestration in lymphoid organs is discussed concerning the discrepancy in this regard between Sjögren's syndrome and SLE, and our understanding of the significance of polyclonal B cell activation in the pathogenesis of these diseases.
通过空斑形成细胞（PFC）试验，同时检测正常个体、系统性红斑狼疮（SLE）患者和原发性干燥综合征患者外周血及骨髓中自发分泌免疫球蛋白（IgG、IgA和IgM）的B细胞数量。正常个体外周血中每10⁶个单核细胞有382（±89）个PFC。无论是活动期还是非活动期的干燥综合征患者，外周血中自发分泌免疫球蛋白的细胞数量均正常（P＞0.2）。相反，非活动期和活动期SLE患者的自发PFC均显著增加（分别为P＜0.05和P＜0.001）。活动期SLE患者的PFC显著多于非活动期SLE患者；每10⁶个单核细胞分别为3984（±960）个和1605（±527）个PFC（P＜0.05）。干燥综合征患者血液中活化B细胞数量未增加，并非是由于活化B细胞优先潴留于骨髓。然而，特别值得关注的是，骨髓不仅是未成熟B细胞的主要来源器官，还是B细胞原位活化或活化B细胞潴留的淋巴器官。与外周血相比，正常个体骨髓中每10⁶个单核细胞的活化B细胞相对增加约20倍，而非活动期和活动期SLE患者骨髓中活化B细胞相对外周血均增加35倍。本文讨论了循环活化B细胞及其在淋巴器官中的潴留与干燥综合征和SLE在这方面差异的潜在相关性，以及我们对这些疾病发病机制中多克隆B细胞活化意义的理解。
