Haynes B F, Metzgar R S, Minna J D, Bunn P A
N Engl J Med. 1981 May 28;304(22):1319-23. doi: 10.1056/NEJM198105283042202.
We studied the surface-antigen pattern of T cells in peripheral blood and cell lines from patients with advanced cutaneous T-cell lymphoma (CTCL) or T-cell acute lymphoblastic leukemia. The antigen patterns of cutaneous T-cell lymphoma cells from peripheral blood and established cell lines were nearly identical; the cells were negative for human thymus antigen (OKT6 and NA1/34), positive for pan-T-cell (OKT3, 17F12, 10.2, and 9.6) and helper-T-cell-subset (OKT4) antigens, and negative for T-cell-subset antigens 3A1 and OKT8. In contrast, the phenotypes of malignant T cells from patients with acute lymphoblastic leukemia were heterogeneous, with at least five patterns of reactivity. The T-cell-specific antibody 3A1 was the only monoclonal reagent that clearly distinguished the peripheral-blood T cells in CTCL (3A1-) from those in acute lymphoblastic leukemia (3A1+). Moreover, 3A1 was the most reliable T-cell marker in acute lymphoblastic leukemia. We conclude that circulating CTCL (Sézary) T cells are homogeneous in their antigen phenotype and are derived from a well-differentiated 3A1-, OKT4+, OKT8- helper-T-cell subset.
我们研究了晚期皮肤T细胞淋巴瘤（CTCL）或T细胞急性淋巴细胞白血病患者外周血及细胞系中T细胞的表面抗原模式。外周血及已建立细胞系的皮肤T细胞淋巴瘤细胞的抗原模式几乎相同；这些细胞对人胸腺抗原（OKT6和NA1/34）呈阴性，对泛T细胞（OKT3、17F12、10.2和9.6）及辅助性T细胞亚群（OKT4）抗原呈阳性，对T细胞亚群抗原3A1和OKT8呈阴性。相比之下，急性淋巴细胞白血病患者恶性T细胞的表型是异质性的，至少有五种反应模式。T细胞特异性抗体3A1是唯一能明确区分CTCL外周血T细胞（3A1阴性）和急性淋巴细胞白血病外周血T细胞（3A1阳性）的单克隆试剂。此外，3A1是急性淋巴细胞白血病中最可靠的T细胞标志物。我们得出结论，循环中的CTCL（Sezary）T细胞在抗原表型上是同质的，且来源于分化良好的3A1阴性、OKT4阳性、OKT8阴性辅助性T细胞亚群。
