通过羊水的细胞学检查确定牛的胎儿性别。
Determination of fetal sex in cattle by cytologic examiantion of amniotic fluid.
作者信息
Bongso T A, Wettimuny S G, Edirisinghe R, Kumaratileke L
出版信息
Am J Vet Res. 1978 Sep;39(9):1545-6.
Amniotic fluid smears (n = 134) of bovine fetuses (69 males, 65 females) at various stages of gestation were stained by the Papanicolaou method. Percentage counts of eosinophilic, orangeophilic, and cyanophilic cells were determined by examining 500 cells per sample. The cyanophilic cell count was significantly (P less than 0.001) higher for the females (37% +/- 3% to 47% +/- 3%) than for the males (19% +/- 2% to 32% +/- 3%). Significant differences, however, were not recorded with the eosinophilic and the orangeophilic cell counts. The percentage cyanophilic count could therefore be used as a method of prenatal sex determination.
采用巴氏染色法对不同妊娠阶段的牛胎儿（69头雄性，65头雌性）羊水涂片（n = 134）进行染色。通过每个样本检查500个细胞来确定嗜酸性、嗜橙色和嗜青色细胞的百分比计数。雌性（37%±3%至47%±3%）的嗜青色细胞计数显著高于雄性（19%±2%至32%±3%）（P<0.001）。然而，嗜酸性和嗜橙色细胞计数未记录到显著差异。因此，嗜青色细胞百分比计数可作为产前性别鉴定的一种方法。
Zotero 插件