[Adenylate cyclase from rat thymus and spleen lymphocytes].

It was found that adenylate cyclase from spleen lymphocytes is more active as compared to that from thymocytes, but is less sensitive to the activating effect of epinephrine and NaF. Adenylate cyclase from different subcellular fractions of thymocytes has different sensitivity to NaF. In the microsomal and mitochondrial fractions the enzyme is inhibited by NaF 7- and 2-fold, while in cell lysate and nuclear-cellular fractions it is activated 3- and 9-fold, respectively. In the presence of NaF adenosine, AMP, PPi and methylene diphosphonic acid inhibit the enzyme activity both in thymus and spleen lymphocytes. Creatine and CrP also significantly decrease the enzyme activity. NH4+ and concanavalin A have no effect and phytohemagglutinin stimulates the enzyme from both sources.