Maron R, Mozes E, Fuchs S, Sela M, Chedid L
Ann Immunol (Paris). 1978 Apr-Jun;129 C(4):489-97.
The effect of a water soluble adjuvant (WSA) extracted from mycobacteria on the immune response to synthetic polypeptides has been studied in rabbits and inbred mice. WSA increased the antibody response of rabbits to the synthetic polypeptide poly(Tyr,Glu)-poly(DLAla)--poly(Lys) designated (T,G)-A--L when injected together with either incomplete or complete Freund's adjuvants (FIA or FCA). As compared to the respective controls, the effect of WSA was more pronounced when injected with FIA. No detectable antibody titre was observed in rabbits immunized with (T,G)-A--L and WSA administered in an aqueous medium. WSA had no enhancing adjuvant effect on the antibody response of C3H.SW high responder mice to (T,G)-A--L when given either with FIA or with FCA. However, the antibody titres of C3H/HeJ and SJL low responder mice to (T,G)-A--L were increased by WSA when injected in combination with FIA or FCA. The immune responses of mice to two other synthetic polypeptides poly(Phe,Glu)-poly(DLAla)--poly(Lys), designated (Phe,G)-A--L, and poly-(Tyr,Glu)-poly(Pro)--poly(Lys), designated (T,G)-Pro--L, were not affected by the addition of WSA. Thus, WSA "corrects" selectively the genetic defect of low responder mice to synthetic antigens.
已在兔和近交系小鼠中研究了从分枝杆菌中提取的一种水溶性佐剂（WSA）对合成多肽免疫反应的影响。当与不完全或完全弗氏佐剂（FIA或FCA）一起注射时，WSA增强了兔对合成多肽聚（酪氨酸，谷氨酸）-聚（D-丙氨酸）-聚（赖氨酸）[命名为（T，G）-A-L]的抗体反应。与各自的对照组相比，当与FIA一起注射时，WSA的效果更明显。在用（T，G）-A-L和WSA在水性介质中给药免疫的兔中未观察到可检测的抗体滴度。当与FIA或FCA一起给予时，WSA对C3H.SW高反应性小鼠对（T，G）-A-L的抗体反应没有增强佐剂作用。然而，当与FIA或FCA联合注射时，WSA可提高C3H/HeJ和SJL低反应性小鼠对（T，G）-A-L的抗体滴度。小鼠对另外两种合成多肽聚（苯丙氨酸，谷氨酸）-聚（D-丙氨酸）-聚（赖氨酸）[命名为（苯丙氨酸，G）-A-L]和聚（酪氨酸，谷氨酸）-聚（脯氨酸）-聚（赖氨酸）[命名为（T，G）-Pro-L]的免疫反应不受WSA添加的影响。因此，WSA选择性地“纠正”了低反应性小鼠对合成抗原的遗传缺陷。
