Taetle R
Blood. 1981 Sep;58(3):653-7.
Cell-free extracts of bone marrow and blood cells from patients with leukemia contain an inhibitor of normal granulocyte/macrophage progenitor (CFU-GM) proliferation (leukemia-associated inhibitory activity, LIA) identified as acidic isoferritins. A comparison was made of the action of crude LIA prepared from frozen-thawed leukemic blood cells and purified spleen ferritin from a patient with chronic myelogenous leukemia, on the proliferation of blast progenitors from patients with acute myelogenous leukemia (AML), and on the promyelocytic leukemia cell line, HL-60. Crude LIA showed no inhibition of blast progenitor or HL-60 proliferation at low concentrations, but inhibited the proliferation of CFU-GM. At higher concentrations, crude LIA inhibited both blast cells and CFU-GM. Purified spleen ferritin failed to inhibit blast progenitors or HL-60 cells at any concentration tested, but inhibited both 70-day and 14-day CFU-GM. Using the thymidine "suicide" technique, the action of LIA was confirmed as being on CFU-GM in S-phase, but it failed to affect the proliferation of blast cell in S-phase. It is concluded that acidic isoferritins inhibit normal CFU-GM but not blast cells from patients with AML. Acidic isoferritins could confer a proliferative advantage of the leukemic clone over its normal counterparts.
白血病患者骨髓和血细胞的无细胞提取物含有一种正常粒细胞/巨噬细胞祖细胞（CFU-GM）增殖抑制剂（白血病相关抑制活性，LIA），已鉴定为酸性异铁蛋白。对从冻融白血病血细胞制备的粗制LIA和来自一名慢性粒细胞白血病患者的纯化脾铁蛋白，在急性髓性白血病（AML）患者的原始祖细胞增殖以及早幼粒细胞白血病细胞系HL-60上的作用进行了比较。粗制LIA在低浓度时对原始祖细胞或HL-60增殖无抑制作用，但抑制CFU-GM的增殖。在较高浓度时，粗制LIA抑制原始细胞和CFU-GM两者。纯化的脾铁蛋白在任何测试浓度下均未能抑制原始祖细胞或HL-60细胞，但抑制70天和14天的CFU-GM。使用胸腺嘧啶核苷“自杀”技术，证实LIA的作用是针对处于S期的CFU-GM，但它未能影响处于S期的原始细胞的增殖。得出的结论是，酸性异铁蛋白抑制AML患者的正常CFU-GM，但不抑制原始细胞。酸性异铁蛋白可赋予白血病克隆相对于其正常对应物的增殖优势。
