胸腺细胞可被刺激产生强烈的痘苗病毒免疫细胞毒性T淋巴细胞反应。

Thymocytes can be stimulated to give a strong vaccinia virus-immune cytotoxic T lymphocyte response.

作者信息

Bennink J R, Doherty P C

出版信息

J Immunol Methods. 1981;43(1):79-85. doi: 10.1016/0022-1759(81)90038-7.

Abstract

The emergence of virus-specific cytotoxic T lymphocyte (CTL) precursors in thymus can be examined directly by stimulating thymocytes for 6 days in irradiated, virus-infected recipients. Responsiveness is a characteristic of the thymocytes themselves and does not reflect the presence of contaminating, blood-bourne T cells. Strong CTL activity may be generated earlier from thymus than from spleen or lymph node of lethally irradiated, bone marrow reconstituted mice. Also, the reconstitution of virus-immune CTL function in these peripheral lymphoid organs is thymus-dependent. Use of this simple experimental protocol offers considerable possibilities for the analysis of the role of the thymus in the ontogeny of the T cell repertoire.

摘要

通过在经辐照的、感染病毒的受体中刺激胸腺细胞6天，可以直接检测胸腺中病毒特异性细胞毒性T淋巴细胞（CTL）前体的出现情况。反应性是胸腺细胞自身的一个特性，并不反映污染的血源性T细胞的存在。与致死性辐照、骨髓重建小鼠的脾脏或淋巴结相比，胸腺可能更早产生强大的CTL活性。此外，这些外周淋巴器官中病毒免疫CTL功能的重建依赖于胸腺。使用这个简单的实验方案为分析胸腺在T细胞库个体发生中的作用提供了相当大的可能性。