Korngold R, Doherty P C
Thymus. 1982 May;4(3):119-33.
The virus-immune responder characteristics of thymocytes, spleen and lymph node (LN) cells from (P1 X P2)F1 leads to P1 radiation chimeras have been examined sequentially at weekly intervals. Adoptively-transferred thymocytes generate strong cytotoxic thymus-derived lymphocyte (CTL) responses from 28 to 100 days after reconstitution with bone marrow, which are almost invariably restricted to recognition of virus presented in the context of P1. This pattern of H-2 restriction is also maintained for spleen and LN cells from the [(H-2kXd)F1 leads to H-2k] and [(H-2kXb)F1 leads to H-2k] combinations but there is random emergence of reactivity to H-2k+virus for peripheral lymphoid cells from [(H-2KkXb)F1 leads to H-2b] chimeras. Treatment of established [(P1 X P2)F1 leads to P1] chimeras with a low dose of cyclophosphamide (Cy) did not lead to the emergence of significant CTL effector function for P2 + virus. Also, administration of a large dose of Cy prior to irradiation of the chimera recipients did not modify the H-2 restriction profile of the chimera, though the level of CTL responsiveness associated with the appropriate H-2 type was apparently enhanced.
对（P1×P2）F1与P1辐射嵌合体的胸腺细胞、脾细胞和淋巴结（LN）细胞的病毒免疫应答特性进行了每周一次的连续检测。过继转移的胸腺细胞在骨髓重建后28至100天产生强烈的细胞毒性胸腺来源淋巴细胞（CTL）应答，这些应答几乎总是局限于识别P1背景下呈现的病毒。对于[（H-2k×d）F1与H-2k]和[（H-2k×b）F1与H-2k]组合的脾细胞和LN细胞，这种H-2限制模式也得以维持，但对于[（H-2Kk×b）F1与H-2b]嵌合体的外周淋巴细胞，对H-2k+病毒的反应性随机出现。用低剂量环磷酰胺（Cy）处理已建立的[（P1×P2）F1与P1]嵌合体，未导致对P2+病毒产生显著的CTL效应功能。此外，在照射嵌合体受体之前给予大剂量的Cy，并未改变嵌合体的H-2限制谱，尽管与适当H-2类型相关的CTL反应性水平明显增强。
