兔肠嗜铬细胞中吲哚胺摄取与储存的放射自显影研究。

Radioautographic study of uptake and storage of indoleamines in the rabbit enterochromaffin cells.

作者信息

Mourre C, Calas A, Gonella J

出版信息

Neurosci Lett. 1981 May 29;23(3):251-6. doi: 10.1016/0304-3940(81)90006-9.

Abstract

After in vitro intra-arterial injection of tritium-labeled 5-hydroxytryptamine or -hydroxytryptophan ([3H]5-HT or [3H]5-HTP) (5 x 10(-7) M) in the presence of cold noradrenaline (5 x 10(-6) M) into rabbit colon, a clear-cut labeling pattern was observed by radioautography. Labelled cells were observed within the mucosa. The labeling was less important after [3H]5-HTP than after [3H]5-HT injection. Ultrastructural study indicates that labeling is confined to the cytoplasm and coincides with polymorphic secretory granules. Hence these labeled cells display not only APUD cells characteristics but also true neuronal properties.

摘要

在冷去甲肾上腺素（5×10⁻⁶M）存在的情况下，将氚标记的5-羟色胺或5-羟色氨酸（[³H]5-HT或[³H]5-HTP）（5×10⁻⁷M）经体外动脉内注射到兔结肠后，通过放射自显影观察到清晰的标记模式。在黏膜内观察到有标记的细胞。注射[³H]5-HTP后的标记比注射[³H]5-HT后的标记程度轻。超微结构研究表明，标记局限于细胞质，且与多形性分泌颗粒一致。因此，这些标记细胞不仅显示出APUD细胞的特征，还具有真正的神经元特性。