Dye E S, North R J, Mills C D
J Exp Med. 1981 Sep 1;154(3):609-20. doi: 10.1084/jem.154.3.609.
The anti-tumor mechanism in mice induced by a subcutaneous injection of syngeneic tumor cells admixed with Corynebacterium parvum was investigated. When mice were implanted in a hind footpad with x 2 1096) tumor cells admixed with 100 microgram C. parvum, the tumor that emerged grew progressively for about 9 d and then underwent progressive and complete regression. It was found that this C. parvum-induced regression was associated with the acquisition of a systemic, T cell-mediated mechanism of immunity to tumor-specific transplantation antigens, which enabled the host to cause the regression of an untreated test tumor growing simultaneously at a distant site. The generation of a C. parvum-potentiated anti-tumor response was dependent on the presence of tumor cells in close association with C. parvum, tumor immunogenicity, and the quantity of tumor antigen in the admixture. The anti-tumor immunity was specific for the tumor in the therapeutic admixture and could be adoptively transferred to normal recipients with Thy-1.2-positive lymphocytes, but not with serum. Complete regression of a distant test tumor by the C. parvum-tumor admixture was limited to tumors below a certain critical size.
研究了皮下注射同基因肿瘤细胞与短小棒状杆菌混合制剂诱导小鼠的抗肿瘤机制。当给小鼠后足垫植入2×10⁶个与100微克短小棒状杆菌混合的肿瘤细胞时，长出的肿瘤大约持续生长9天，然后逐渐完全消退。发现这种短小棒状杆菌诱导的消退与获得针对肿瘤特异性移植抗原的全身性、T细胞介导的免疫机制有关，这使宿主能够使同时在远处生长的未经处理的试验肿瘤消退。短小棒状杆菌增强的抗肿瘤反应的产生取决于与短小棒状杆菌紧密相关的肿瘤细胞的存在、肿瘤免疫原性以及混合物中肿瘤抗原的量。抗肿瘤免疫对治疗混合物中的肿瘤具有特异性，并且可以通过Thy-1.2阳性淋巴细胞而不是血清将其过继转移给正常受体。短小棒状杆菌-肿瘤混合物使远处试验肿瘤完全消退仅限于一定临界大小以下的肿瘤。
