Korkina L G, Dobretsov G E, Walzel G, Kogan E M
J Immunol Methods. 1981;45(3):227-37. doi: 10.1016/0022-1759(81)90301-x.
Non-fixed lymphocytes of rats and mice were stained with the membrane fluorescent probe, 3-methoxybenzanthrone. The probe is capable of binding preferentially with lymphocyte membranes and fluoresces in the green spectral region. Microfluorometry of single cells showed that lymphocytes differ in all lymphoid organs and these may be a 3-10-fold variation in fluorescence intensity. Lymphocytes can be divided into two groups according to fluorescence intensity: "bright" and "dim". The proportions of "bright" and "dim" cells were determined in rats and mice for various lymphoid organs in the normal state, after thymectomy and cyclophosphamide treatment, and also after lymphocyte separation on a nylon wool column. In all cases the proportion of bright lymphocytes corresponded to the B-cell content, and the proportion of dim lymphocytes corresponded to the content of T-cells.
用膜荧光探针3-甲氧基苯并蒽对大鼠和小鼠的非固定淋巴细胞进行染色。该探针能够优先与淋巴细胞膜结合，并在绿色光谱区域发出荧光。单细胞显微荧光测定表明，淋巴细胞在所有淋巴器官中存在差异，荧光强度可能有3至10倍的变化。根据荧光强度，淋巴细胞可分为两组：“亮”细胞和“暗”细胞。测定了正常状态下、胸腺切除术后、环磷酰胺处理后以及在尼龙毛柱上分离淋巴细胞后，大鼠和小鼠各种淋巴器官中“亮”细胞和“暗”细胞的比例。在所有情况下，亮淋巴细胞的比例与B细胞含量相对应，暗淋巴细胞的比例与T细胞含量相对应。
