Barker R H, O'Shea P F, Strauss P R
J Cell Physiol. 1981 Nov;109(2):243-51. doi: 10.1002/jcp.1041090207.
It is generally assumed that lymphocytes do not adhere firmly to solid substrata. However, in attempting to culture murine spleen and thymus cells in RPMI 1640 without serum, we observed that some cells adhered to glass or plastic surfaces. As a minimum estimate. 10-12% of the applied spleen cells and 22% of those from thymus attached between 1 and 24 hours after plating. The cells remained attached despite extensive and vigorous washing. Viability of 70% was maintained between 4 hours and 3 days in culture. Readdition of 10% mouse or horse serum for 2 hours resulted in removal of 80% of the attached cells. The percentage of adherent cells was not affected by cell density, but was greatly reduced when cells were cultured at 4 degrees C. Glutaraldehyde-fixed cells did not adhere. Adherent cells were primarily T lymphocytes. The cell-plate distance would indicate a focal contact mode of adherence; however, the absence of filamentous material at the adherent surface and the broad, continuous surface apposition would imply a close contact mode. We conclude that attachment modes described for fibroblasts in culture are not applicable for lymphocytes.
一般认为淋巴细胞不会牢固地黏附于固体基质。然而，在尝试用不含血清的RPMI 1640培养基培养小鼠脾脏和胸腺细胞时，我们观察到一些细胞黏附于玻璃或塑料表面。据最低估计，接种后1至24小时内，所接种的脾脏细胞中有10 - 12%以及胸腺细胞中有22%黏附。尽管经过大量剧烈冲洗，这些细胞仍保持黏附状态。培养4小时至3天期间，细胞活力维持在70%。重新添加10%的小鼠或马血清2小时后，80%的黏附细胞被去除。黏附细胞的百分比不受细胞密度影响，但当细胞在4℃培养时则大幅降低。经戊二醛固定的细胞不发生黏附。黏附细胞主要是T淋巴细胞。细胞与培养板之间的距离表明其黏附方式为局部接触模式；然而，黏附表面不存在丝状物质以及广泛、连续的表面贴合则意味着是紧密接触模式。我们得出结论，培养成纤维细胞时所描述的黏附模式不适用于淋巴细胞。
