Felts C, Engel D, Ammons W, Narayanan A S
J Periodontol. 1981 Oct;52(10):603-8. doi: 10.1902/jop.1981.52.10.603.
Unfractionated peripheral blood lymphocytes (PBL) and the T and B cell fractions of PBL from four patients who had received scleral allografts, and fractions from four control subjects were tested in vitro for proliferative responsiveness to collagen and glycosaminoglycan-enriched extracts of allogeneic sclera. None of the unfractionated lymphocyte cultures (patients and controls) were responsive to the scleral extracts. However, B-enriched lymphocyte cultures from one patient and two control subjects did respond to the scleral extracts, although there was considerable individual variation in the magnitudes of these responses (stimulation indices ranging from 1.5 to 11.5). In contrast, the DNA-synthesis response of T cells to scleral extracts was strongly suppressed. Furthermore, T cells pre-exposed for 24 hours to scleral collagen were capable of suppressing B-cell responsiveness to mitogens in autologous mixed lymphocyte cultures. Our findings confirm the lack of apparent adverse immunologic responsiveness to scleral allografts in humans as reported by others. It is possible that immunological tolerance to the scleral allografts is due to a suppressor T-cell mechanism which is triggered by scleral collagen.
对4例接受巩膜同种异体移植患者的未分级外周血淋巴细胞（PBL）及其PBL中的T细胞和B细胞组分，以及4例对照受试者的细胞组分进行体外检测，观察其对同种异体巩膜富含胶原蛋白和糖胺聚糖提取物的增殖反应性。所有未分级淋巴细胞培养物（患者和对照）对巩膜提取物均无反应。然而，1例患者和2例对照受试者的富含B细胞的淋巴细胞培养物对巩膜提取物有反应，尽管这些反应的强度存在相当大的个体差异（刺激指数范围为1.5至11.5）。相比之下，T细胞对巩膜提取物的DNA合成反应受到强烈抑制。此外，预先与巩膜胶原蛋白接触24小时的T细胞能够抑制自体混合淋巴细胞培养物中B细胞对有丝分裂原的反应性。我们的研究结果证实了其他人所报道的人类对巩膜同种异体移植缺乏明显不良免疫反应性。对巩膜同种异体移植的免疫耐受可能是由于巩膜胶原蛋白触发的抑制性T细胞机制所致。
