Dyminski J W
Thymus. 1981 Nov;3(4-5):231-55.
We have previously shown that myeloma-derived or serum-derived immunoglobulin could facilitate in vitro alloantigen recognition by immunocompetent thymus cells ('subset-I thymocytes'). The present report demonstrates the isolation of surface immunoglobulins from radiolabeled splenic B lymphocytes. Solubilized radiolabeled preparations of surface immunoglobulin are shown to bind in vitro to lymphoid cells, especially to immunocompetent thymus cells. Immunocompetent thymus cells (subset-I thymocytes preferentially bind more IgG than IgM. Papain digests of IgG are also bound by subset-I thymocytes; preferential binding of the Fc piece is observed. Although the binding of IgG or its pieces per se does not activate lymphoid cells in vitro a role for the binding of IgG during alloantigen recognition in vitro between immunocompetent thymus cells is demonstrated.
我们之前已经表明，骨髓瘤来源或血清来源的免疫球蛋白能够促进免疫活性胸腺细胞（“I 亚群胸腺细胞”）在体外对同种异体抗原的识别。本报告展示了从放射性标记的脾 B 淋巴细胞中分离表面免疫球蛋白的过程。经溶解的放射性标记表面免疫球蛋白制剂在体外显示出与淋巴细胞结合，尤其是与免疫活性胸腺细胞结合。免疫活性胸腺细胞（I 亚群胸腺细胞）优先结合的 IgG 比 IgM 更多。IgG 的木瓜蛋白酶消化产物也能被 I 亚群胸腺细胞结合；观察到对 Fc 片段的优先结合。尽管 IgG 或其片段本身的结合在体外不会激活淋巴细胞，但已证明 IgG 的结合在免疫活性胸腺细胞体外同种异体抗原识别过程中发挥作用。
