Felix E, Katz S, Teodorescu A, Teodorescu M
J Surg Oncol. 1981;18(4):323-9. doi: 10.1002/jso.2930180401.
Peripheral blood lymphocyte counts were determined in 57 breast clinic patients. These patients were grouped into four clinical/pathological groups: 18 had benign breast disease, 13 had a history of breast cancer but were free of disease at the time of the study, 7 had a history of breast cancer and were free of disease at the time of the study but were on adjuvant chemotherapy, and 19 had active metastatic breast cancer. Two parameters were investigated in a double blind study: 1) the absolute lymphocyte counts and 2) the percentages of lymphocytes that bound different bacteria as markers of lymphocyte subpopulations in conventionally stained blood smears. A significant reduction in mean lymphocyte counts was demonstrated in patients with advanced disease. A significant increase in these counts was found in patients who were free of disease following surgical treatment alone. The T1T2 cells (our denomination), the subpopulation of cells responsible for specific killing in vitro and for suppression of natural cytotoxic cells, was significantly reduced in patients with advanced disease under treatment. The T/B cell ratio in all groups was the same and within normal range. These observations suggest that the observed decrease in the total lymphocytes is due not only to an overall decrease in all classes of lymphocytes but especially in those responsible for specific cell-mediated reactions. They also show that bacteria can be used as reliable reagents for the identification of lymphocyte subpopulations in blood smears.
对57名乳腺门诊患者进行了外周血淋巴细胞计数。这些患者被分为四个临床/病理组：18例患有良性乳腺疾病，13例有乳腺癌病史但在研究时无疾病，7例有乳腺癌病史且在研究时无疾病但正在接受辅助化疗，19例有活动性转移性乳腺癌。在一项双盲研究中对两个参数进行了调查：1）绝对淋巴细胞计数和2）在传统染色血涂片中结合不同细菌作为淋巴细胞亚群标志物的淋巴细胞百分比。晚期疾病患者的平均淋巴细胞计数显著降低。仅接受手术治疗后无疾病的患者中这些计数显著增加。在接受治疗的晚期疾病患者中，负责体外特异性杀伤和抑制自然细胞毒性细胞的细胞亚群T1T2细胞（我们的命名）显著减少。所有组的T/B细胞比值相同且在正常范围内。这些观察结果表明，观察到的总淋巴细胞减少不仅是由于所有类别的淋巴细胞总体减少，尤其是负责特异性细胞介导反应的淋巴细胞减少。它们还表明，细菌可用作血涂片中淋巴细胞亚群鉴定的可靠试剂。
