血红素a及其衍生物的磁圆二色性研究。
Magnetic-circular-dichroism studies of haem a and its derivatives.
作者信息
Brittain T, Greenwood C, Springall J P, Thomson A J
出版信息
Biochem J. 1978 Aug 1;173(2):411-7. doi: 10.1042/bj1730411.
Abstract
The low-temperature m.c.d.(magnetic-circular-dichroism) spectra of haem a derivatives are presented. It is shown that reduced haem a in pyridine contains the low-spin ferrous ion and that oxidized haem a in the presence of excess imidazole has the low-spin ferric form. Both the m.c.d. and the absorption spectra of haem a in excess pyridine are changed by the addition of excess sodium dithionite into a form similar to that of haems b and c. This is interpreted in terms of an attack, by reduced pyridine, at the 8-formyl group of the haem a ring. This leads to a partial assignment of the electronic spectrum of haem a.
摘要
本文给出了血红素a衍生物的低温磁圆二色性（m.c.d.）光谱。结果表明，吡啶中的还原型血红素a含有低自旋亚铁离子，而在过量咪唑存在下的氧化型血红素a具有低自旋铁离子形式。向过量吡啶中的血红素a加入过量连二亚硫酸钠后，其m.c.d.光谱和吸收光谱均发生变化，变为与血红素b和c相似的形式。这可以用还原吡啶对血红素a环的8-甲酰基的攻击来解释。这导致了对血红素a电子光谱的部分归属。
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