Graves C, Sachs G
Biochim Biophys Acta. 1982 Feb 8;685(1):27-31. doi: 10.1016/0005-2736(82)90030-x.
The carbocyanine dye, diS-C3-(5) was used to quantitate the plasma membrane potential of the bullfrog corneal endothelium. It was shown that valinomycin hyperpolarized the endothelial cell and that in the presence of the ionophore the membrane potential largely reflected the K+ equilibrium potential. Using calibration curves constructed by changing medium K+ concentration in the presence of valinomycin, and nigericin and ouabain to abolish ion gradients and electrogenic pump activity, the cell membrane potential was calculated to be 28.6 +/- 4.2 mV. The major source of this potential was a K+ diffusion potential, and the membrane Na+ conductance reduced the cell potential to less than the apparent K+ equilibrium potential of 51.5 +/- 5.1 mV. About 20% of the cell potential could be ascribed to the rheogenic (Na+ + K+)-ATPase.
采用羰花青染料二辛可宁酸三碳菁（diS-C3-(5)）对牛蛙角膜内皮细胞质膜电位进行定量分析。结果表明，缬氨霉素可使内皮细胞超极化，且在离子载体存在的情况下，膜电位在很大程度上反映了钾离子平衡电位。利用在缬氨霉素存在下通过改变培养基钾离子浓度构建的校准曲线，并使用尼日利亚菌素和哇巴因消除离子梯度和电生泵活性，计算出细胞膜电位为28.6±4.2 mV。该电位的主要来源是钾离子扩散电位，膜钠离子电导使细胞电位降低至低于51.5±5.1 mV的表观钾离子平衡电位。约20%的细胞电位可归因于生电型（钠离子+钾离子）-ATP酶。
