对化学诱导的大鼠神经源性肿瘤的天然细胞毒性。

Natural cytotoxicity against chemically induced neurogenic rat tumors.

作者信息

De Bernardo E

出版信息

Exp Cell Biol. 1982;50(1):39-55. doi: 10.1159/000163125.

Abstract

The 51Cr cytotoxicity assay was modified for ethylnitrosourea (ENU) and methylnitrosourea (MNU) induced rat neurogenic tumor cell lines. Investigations on "immune" lymphoid cytotoxicity showed little cytotoxicity above normal. Alteration in the level of lymphocytotoxicity was seen immediately after injection of a highly immunogenic tumor (EA-528). Natural cytotoxicity (NC) was present against all neurogenic tumor cell lines tested and xenogenic cell lines. The level of NC activity was dependent on age, specific tumor cell line and could be increased by isolating the nylon wool non-adherent "T" cell population. Allogenic WFu rat lymphocytes were tested for NC activity in vitro against CDF tumor cells. NC activity has potential use against these tumors in vivo.

摘要

针对乙基亚硝基脲（ENU）和甲基亚硝基脲（MNU）诱导的大鼠神经源性肿瘤细胞系，对51Cr细胞毒性测定法进行了改良。对“免疫”淋巴细胞毒性的研究表明，其细胞毒性略高于正常水平。在注射高度免疫原性肿瘤（EA - 528）后，立即观察到淋巴细胞毒性水平的变化。对所有测试的神经源性肿瘤细胞系和异种细胞系均存在自然细胞毒性（NC）。NC活性水平取决于年龄、特定肿瘤细胞系，并且通过分离尼龙毛非黏附性“T”细胞群体可使其增加。对同种异体WFu大鼠淋巴细胞进行体外测试，以检测其对CDF肿瘤细胞的NC活性。NC活性在体内对这些肿瘤具有潜在的应用价值。