Stavrou D, Bilzer T, Anzil A P, Hultén M, Kaldrymidou E
Anticancer Res. 1981;1(3):125-34.
Brain tumors were induced in inbred Fischer rats (F344) by administration of methylnitrosourea in the drinking water. One of the induced tumors, a pleomorphic glioma (78-219), was established in vitro and propagated as 78FR-G-219 permanent cell line. The tumorigenicity of the established line was investigated by intracerebral or subcutaneous inoculation of 1X10 (6) - 10X10(6) cells. Lymphocytes infiltrating secondary tumors (TIL) were enriched by a single step centrifugation on different discontinuous Percoll density gradients, while blood lymphocytes (PBL) and lymphocytes from spleen and lymph node (SL and LNL) were enriched by Ficoll - Isopaque flotation. The reactivity spectrum of the isolated lymphocytes raised against cultured 78FR-G-219 cells was monitored by means of two different assays: Lymphocyte microcytotoxicity test (LMC) and colony inhibition assay (CIA). Reactivity of PBL, SL and LNL of glioma-bearing animals was clearly reduced, while TIL showed no natural killer (NK) activity, no cytotoxicity against syngeneic 78FR-G-219 pleomorphic glioma cells and no colony inhibition in mixed lymphocyte/target cell cocultivation. NK activity of TIL was only slightly reduced against target cells of a non-cross-reacting syngeneic astrocytoma line (78FR-G-299).
通过在饮用水中给予甲基亚硝基脲，在近交系Fischer大鼠（F344）中诱发脑肿瘤。其中一个诱发的肿瘤，一种多形性胶质瘤（78 - 219），在体外建立并作为78FR - G - 219永久细胞系进行传代培养。通过脑内或皮下接种1×10⁶ - 10×10⁶个细胞来研究该建立细胞系的致瘤性。通过在不同的不连续Percoll密度梯度上进行单步离心富集浸润继发性肿瘤的淋巴细胞（TIL），而通过Ficoll - Isopaque漂浮法富集血液淋巴细胞（PBL）以及来自脾脏和淋巴结的淋巴细胞（SL和LNL）。通过两种不同的检测方法监测分离出的淋巴细胞对培养的78FR - G - 219细胞产生的反应谱：淋巴细胞微细胞毒性试验（LMC）和集落抑制试验（CIA）。荷胶质瘤动物的PBL、SL和LNL的反应性明显降低，而TIL没有自然杀伤（NK）活性，对同基因的78FR - G - 219多形性胶质瘤细胞没有细胞毒性，并且在混合淋巴细胞/靶细胞共培养中没有集落抑制作用。TIL对非交叉反应的同基因星形细胞瘤系（78FR - G - 299）的靶细胞的NK活性仅略有降低。
